Ishwar Radhakrishnan Professor

Research Summary:

The Radhakrishnan lab studies the molecular mechanisms of transcription regulation, specifically, how DNA-bound transcription factors recruit coactivators, nucleating protein-protein interaction networks that extend to the transcription machinery, and how chromatin-modifying complexes read, interpret, and modify the histone code. We are asking these questions in the context of (i) cAMP signaling mediated by CREB and its coactivators and (ii) a cohort of related, yet functionally distinct, histone deacetylase-associated chromatin-modifying complexes.

Selected Publications:

Molecular Basis for the Mechanism of Constitutive CBP/p300 Coactivator Recruitment by CRTC1-MAML2 and Its Implications in cAMP Signaling. Clark MD, Kumar GS, Marcum R, Luo Q, Zhang Y, and Radhakrishnan I. Biochemistry. 2015 September 8;54(35):5439-5446.

Structural insights into the assembly of the histone deacetylase-associated Sin3L/Rpd3L corepressor complex. Clark MD, Marcum R, Graveline R, Chan CW, Xie T, Chen Z, Ding Y, Zhang Y, Mondragón A, David G, and Radhakrishnan I. PNAS. 2015 July 14;112(28):E3669-E3678.

Structural Basis for Multi-specificity of MRG Domains. Xie T, Zmyslowski AM, Zhang Y, and Radhakrishnan I. Structure. 2015 June 2;23(6):1049-1057.

A Role for WDR5 in Integrating Threonine 11 Phosphorylation to Lysine 4 Methylation on Histone H3 during Androgen Signaling and in Prostate Cancer. Kim J-Y, Banerjee T, Vinckevicius A, Luo Q, Parker JB, Baker MR, Radhakrishnan I, Wei J-J, Barish GD, and Chakravarti D. Molecular Cell. 2014 May 22; 54(4):613-625.

Mechanism of CREB recognition and coactivation by the CREB-regulated transcriptional coactivator CRTC2. Luo Q, Viste K, Urday-Zaa JC, Kumar GS, Tsai W-W, Talai A, Mayo KE, Montminy M, and Radhakrishnan I. PNAS. 2012 December 18;109(51):20865-20870.

Sequence Requirements for Combinatorial Recognition of Histone H3 by the MRG15 and Pf1 Subunits of the Rpd3S/Sin3S Corepressor Complex. Kumar GS, Chang W, Xie T, Patel A, Zhang Y, Wang GG, David G, and Radhakrishnan I. Journal of Molecular Biology. 2012 September 28;422(4):519-531.

Structural Basis for Molecular Interactions Involving MRG Domains: Implications in Chromatin Biology. Xie T, Graveline R, Kumar GS, Zhang Y, Krishnan A, David G, and Radhakrishnan I. Structure. 2012 January 11;20(1):151-160.

Structure of the 30-kDa Sin3-associated Protein (SAP30) in Complex with the Mammalian Sin3A Corepressor and Its Role in Nucleic Acid Binding. Xie T, He Y, Korkeamaki H, Zhang Y, Imhoff R, Lohi O, and Radhakrishnan I. Journal of Biological Chemistry. 2011 August 5;286(31):27814-27824.

Solution Structure of the mSin3A PAH2–Pf1 SID1 Complex: A Mad1/Mxd1-Like Interaction Disrupted by MRG15 in the Rpd3S/Sin3S Complex. Kumar GS, Xie T, Zhang Y, and Radhakrishnan I. Journal of Molecular Biology. 2011 May 20;408(5):987-1000.

Selected Honors:

Jane Coffin Childs Memorial Fund for Medical Research Fellow, 1995-1998

Basil O’Connor Starter Scholar of the March of Dimes Birth Defects Foundation, 2001-2002

Scholar of the Leukemia and Lymphoma Society, 2004-2009